Vaccin culture and the like and the preparation thereof.



CHARLES JULES HENRI NICOLLE, OF TUNIS, TUNIS.

VACCIN CULTURE AND THE LIKE AND THE PREPARATION THEREOF.

\1 No Drawing.

To all whom it may concern:

Be it known that T, CHARLES JULEs HENRI NICOLLE, citizen of the Republicof France,

residing at Institut Pasteur de Tunis, Tunis,

Tunis, have invented certain new and useful Improvements in and Relatingto Vaccin Cultures and the like and the Preparation Thereof, of whichthe following is a specification.

In order to obtain a vaccin (e. g. for preventive and therapeuticpurposes) of the maximum immunizing power from bacterial cultures orfrom killed virus, it is essential to destroy the vitality .of thebacillus without violently coagulating its protoplasm and preventing allsubsequent autolysis.

To this end, according to the invention we stabilize the vaccin bya'fluorin compound. Example: The virus or bacterial culture isemulsified in a solution of sodium fluorid (0.7 per cent, though thisstrength is only given by way of example). The emulsion is carefullycentrifuged. The deposited matter is washed and then suspended in thesame fiuorid solution. The bacterial mass is separated by centrifugingat a suitable speed and the fine emulsion of bacteria so isolatedis-separated, and when brought to the optimum bacterial titration thisyields the stabilized vaccin.

The sodium fluorid may be replaced by other soluble fiuorids, doublesalts of fluorin 0r fluosels.

At the moment ,of using the vaccin the stabilizing agent maybe renderedinsoluble by a suitable salt, if desired.

It is of course to be understood in construing my claims that vaccinsare distin- Specification of Letters Patent.

Application fiIed December 13, 1913.

Patented @ct. 24L, Twit Serial No. 806,589.

guished from sera by the fact that vaccins contain bacteria.

.I declare that what it claim is 1. A process for producing vaccins,comprising forming a bacterial culture, treating the same with a fluoridcompound, centrifuging the emulsion, washing out the de posited matter,suspending the same in the samefiuorid solution, then separating thebacterial mass by centrifuging at a suitable speed and then separatingthe fine emulsion of bacteria and bringing the same to the optimumbacterial titration for producing stabilized vaccin. w

2. A process for producing vaccins, comprising forming abacterialculture and stabilizing the same by treatment with a soluble fiuoridcompound to prevent autolysis.

3. A process for producingvaccins, comprising forming a bacterialculture "and treating the same with a solution containing sodiumfluorid.

4. A process vfor producing vaccins, comprising forming a bacterialcultureand,

treating the same with a solution containing sodium fluorid, andrendering the stabilizing agent insoluble when the vaccin is to be used.

